NGS laboratory is mainly used for high-throughput sequencing detection. Today's article mainly introduces the regional division of NGS laboratory design, including the three parts of partition, function and instrument. Please pay more attention to it.

Regional division of NGS laboratory design

1. Reagent preparation area

(1) Regional function: used for the preparation, distribution and storage of reagents required for experimental projects.

(2) Equipment equipment: It must be equipped with a sampler, refrigerator, analytical balance, micro-table centrifuge, vortex oscillator, pipette, movable UV lamp, ultra-clean workbench and other equipment.

2. Specimen and library preparation area

(1) Regional functions: used for blood processing, plasma preparation, DNA extraction, DNA fragmentation, DNA sample purification, DNA concentration detection, end repair, adapter connection, and sample sequencing library construction.

(2) Equipment equipment: it must be equipped with a refrigerated centrifuge, a micro benchtop centrifuge, a handheld three-tube centrifuge, a vortex shaker, a vertical mixer, a pipette, a movable UV lamp, a biological safety cabinet, a refrigerator, a tray balance, Qubit 2.0 nucleic acid quantifier, computer (connected to the Internet), barcode printer, ordinary printer, scanner, magnetic stand and other equipment.

3. Library amplification and detection area

(1) Regional function: used for PCR amplification and purification after library construction, library concentration detection, library mixing and quality inspection analysis after mixing.

(2) Equipment equipment: must be equipped with PCR instrument, QPCR instrument, Qubit2.0 nucleic acid quantifier, movable ultraviolet lamp, ultra-clean workbench, refrigerator, micro bench centrifuge, vortex oscillator, UPS, refrigerator, pipette , Magnetic stand and other equipment.

4. Sequencing area

(1) Regional function: used for sequencing template preparation, such as emulsion PCR and magnetic bead enrichment recovery, as well as on-machine sequencing, data analysis, report review and distribution.

(2) Equipment equipment: must be equipped with high-throughput sequencer, PC computer, printer, UPS, constant temperature metal bath, pure water meter, refrigerator, micro benchtop centrifuge, vortex oscillator, pipette, ultra-clean bench, Movable UV lamp, magnetic stand, PCR instrument and other equipment.