Regional division is one of the important links in the design of the cell laboratory, so what are the main areas of the cell laboratory? Today's article will introduce the four major areas of the cell laboratory in detail, and I hope it will be helpful to everyone.

The four major areas designed by the cell laboratory are usually divided into four areas when designing the cell laboratory, namely: reagent storage and preparation area, specimen preparation area, amplification reaction mixture preparation and amplification area, and amplification products Analysis area.

Area one, reagent storage and preparation area

Function: The reagent storage and preparation area of the cell laboratory has three functions, which are the preparation of storage reagents, the dispensing of reagents, and the preparation of the main reaction mixture.

Note: The materials used in the experimental project, such as reagents and reagent raw materials, specimens and specimen raw materials, etc., must be stored in this area. As for whether the airflow pressure needs to be controlled, there are no strict requirements in this area.

Area two. Specimen preparation area

Function: The specimen preparation area of the cell laboratory also has three functions, namely the preservation of clinical specimens, the extraction and storage of nucleic acids (RNA and DNA), the addition of nucleic acids to the amplification reaction tube and the synthesis of DNA when measuring RNA.

Note: In order to prevent the air in the adjacent area from entering this area, the pressure gradient should be set to a positive pressure compared to the adjacent area. When carrying out the operation of the experimental project, the staff should avoid unnecessary walking to prevent aerosol pollution during the sample addition operation.

Area three, amplification reaction mixture preparation and amplification area

Function: The amplification reaction mixture preparation and amplification zone of the cell laboratory have two functions, namely DNA amplification and making the prepared experimental reagents into the reaction mixture.

Note: The pollution index of the experimental amplification is relatively high, and the pressure gradient should be set to negative pressure compared with the adjacent area. The advantage of this is that it can effectively prevent aerosol leakage from this area.

Area four, amplification product analysis area

Function: The amplification product analysis area of the cell laboratory has one function, which is to measure the relevant amplified fragments.

Note: If the fully automatic closed analytical instrument has been preset during the design of the cell laboratory, then there is no need to divide this area. The pressure gradient in this area should be set to negative pressure compared to the adjacent area. The advantage of this is that it can effectively prevent the amplification product from spreading to other areas.

Finally, if you want to know more about laboratory design, you might as well click "Technical Support" to view it.