The second-generation sequencing NGS laboratory is a laboratory for genetic testing (high-throughput sequencing detection). The focus of the construction of the second-generation sequencing NGS laboratory is mainly in the design of layout partitions, structural materials, air pressure adjustment, disinfection, and lighting. Several aspects of the transfer window.

I,The design layout of the first and second-generation sequencing NGS laboratories

The design of the second-generation sequencing NGS laboratory is divided into 4 areas, namely: reagent preparation area, specimen and library preparation area, library amplification and detection area, and sequencing area.

1. Reagent preparation area

The regional functions and equipment of the reagent preparation area of NGS laboratory are as follows:

(1) Regional function: used for the preparation, distribution and storage of reagents required for experimental projects.

(2) Equipment: It must be equipped with a sampler, refrigerator, analytical balance, micro-table centrifuge, vortex oscillator, pipette, movable UV lamp, ultra-clean bench and other equipment.

2. Specimen and library preparation area

The regional functions and equipment of the NGS laboratory specimen and library preparation area are as follows:

(1) Regional functions: used for blood processing, plasma preparation, DNA extraction, DNA fragmentation, DNA sample purification, DNA concentration detection, end repair, adapter connection, and sample sequencing library construction.

(2) Equipment equipment: it must be equipped with a refrigerated centrifuge, a micro benchtop centrifuge, a handheld three-tube centrifuge, a vortex shaker, a vertical mixer, a pipette, a movable UV lamp, a biological safety cabinet, a refrigerator, a tray balance, Qubit 2.0 nucleic acid quantifier, computer (connected to the Internet), barcode printer, ordinary printer, scanner, magnetic stand and other equipment.

3. Library amplification and detection area

The regional functions and equipment of the library amplification and detection area of NGS laboratory are as follows:

(1) Regional function: used for PCR amplification and purification after library construction, library concentration detection, library mixing and quality inspection analysis after mixing.

(2) Equipment equipment: must be equipped with PCR instrument, QPCR instrument, Qubit2.0 nucleic acid quantifier, movable UV lamp, ultra-clean workbench, refrigerator, micro bench centrifuge, vortex oscillator, UPS, refrigerator, pipette , Magnetic stand and other equipment.

4. Sequencing area

The regional functions and equipment of the sequencing area of the NGS laboratory are as follows:

(1) Regional function: used for sequencing template preparation, such as emulsion PCR and magnetic bead enrichment recovery, as well as on-machine sequencing, data analysis, report review and issuance.

(2) Equipment equipment: must be equipped with high-throughput sequencer, PC computer, printer, UPS, constant temperature metal bath, pure water meter, refrigerator, micro-desktop centrifuge, vortex oscillator, pipette, ultra-clean bench, Movable UV lamp, magnetic stand, PCR instrument and other equipment.

II. Main structural materials of NGS laboratory for next-generation sequencing

The design and decoration of the second-generation sequencing NGS laboratory should use color steel plates and aluminum alloy materials as the main building materials. The internal corners and external corners of the interior are made of aluminum alloy 50 inner round aluminum. The advantage of this is that the internal structure is firm, the sealing is good, and it is not easy. Pollution and dust accumulation.

III. Air pressure adjustment in NGS laboratory for next-generation sequencing

In the second-generation sequencing NGS laboratory, air pressure adjustments must be installed on the outer walls and doors of the experimental area with adjustable air return vents, and air is ventilated into the room through the return air vents.

IV. Disinfection and lighting of NGS laboratories

In terms of disinfection, the experimental area, the top of the buffer zone, and the inside of the transmission window of the NGS laboratory of the second-generation sequencing should be equipped with ultraviolet lamps for disinfection. If you want to disinfect the experimental table locally, you must also be equipped with a mobile ultraviolet lamp; in terms of lighting, the lighting equipment must be dust-free and easy to clean. Here, SAREN Sanren recommends to be equipped with purification lamps.

V. Delivery window of NGS laboratory for next-generation sequencing

The delivery window is divided into mechanical linkage type and electronic linkage type. It is recommended to equip the former for the design and decoration of the NGS laboratory of second-generation sequencing to ensure that the samples and reagents will not be contaminated during the delivery process.