The ideal plant tissue culture room should be set up in a quiet, clean, and away from pollution, preferably in the upwind direction of the main wind direction throughout the year, which will better control pollution. When we build it, apart from considering the issues of funding and experimental nature, we must also take into account the principles of science, efficiency, economy and practicality. These principles can also be projected on the internal structure of our tissue culture room. Planning and design is particularly important at this time. Good designers will be more thoughtful in terms of internal design function layout. The layout of functional rooms is undoubtedly an important part of the plant cell culture laboratory design. So, how can we do a good job in the layout of functional rooms designed for plant cell culture laboratories? CEIDI has compiled relevant knowledge for everyone and looks forward to sharing it with you. As an important branch of biological laboratory decoration, the plant cell laboratory has high requirements for cleanliness. The cell laboratory building structure usually includes two parts: the basic laboratory (that is, the sterilization laboratory, the preparation room, the sterile operating room) and the auxiliary laboratory (the cytology laboratory, the biochemical analysis room, the photography room, and the dark room). The graphic design area of the cell laboratory is mainly divided into six areas according to the work content: aseptic operation, incubation, preparation, cleaning, disinfection and sterilization, and storage.

When designing a plant cell culture laboratory, the functional rooms must be divided into 7 categories: aseptic operation room, medium configuration room, buffer room, washing room, disinfection room, culture room, and cytology identification room.

Function room 1: Aseptic operation room

The aseptic operation room of the plant cell culture laboratory is used for sample inoculation operation, so it is also called "inoculation room". Since it is an aseptic operation, measures must be taken to prevent all kinds of pollution. Specifically, it is necessary to set up a buffer room, and its door opening direction is staggered with the direction of the inoculation room, and cannot be opened at the same time.

Function room 2: Medium configuration room

The medium configuration room of the plant cell culture laboratory should be equipped with balances, microwave ovens, electric stoves, refrigerators, instrument cabinets, medicine cabinets, suction pumps, pH meters, medium dispensers, culture bottles of various specifications, petri dishes, measuring cylinders, and flasks , Volumetric flasks, storage bottles, pipettes and other equipment.

Function Room 3: Buffer Room

The buffer room of the plant cell culture laboratory should be equipped with sinks, ultraviolet lamps, lockers, ultra-clean workbenches, dissecting mirrors and other equipment. In addition, the wall of the buffer room should be made of ceramic tile or plastic steel plate.

Function Room 4: Washing Room

The washing room of the plant cell culture laboratory should be equipped with special washing tanks, bottle washing machines, washing liquid tanks, falling racks, drying boxes, sample cabinets, ultrasonic cleaners and other equipment. In addition, the floor design of the washing room should be considered from the three aspects of non-slip, moisture resistance and drainage.

Function Room 5: Disinfection Room

The disinfection room of the plant cell culture laboratory should be equipped with electric furnace, test bench, autoclave, dry heat disinfection cabinet, exhaust fire extinguishing equipment, bacterial filtration equipment and other instruments and equipment.

Function Room 6: Training Room

The culture room of the plant cell culture laboratory should be equipped with culture racks, turntables, shakers, light incubators, biochemical incubators, automatic timers and other equipment. In addition, the cultivation room has strict requirements on temperature and light, so the room cannot be installed with windows, but with exhaust fans. The floor should be made of terrazzo or ceramic tiles.

Function Room 7: Cytology Identification Room

The cytology identification room of the plant cell culture laboratory shall be equipped with microscopes, camera systems and other equipment. And when designing the laboratory, it should be considered from three aspects: clean, bright and dry.