As an important branch of biological laboratory decoration, the cell laboratory has high requirements for cleanliness. The building structure of the cell laboratory usually includes two parts: the basic laboratory (that is, the disinfection laboratory, the preparation room, the sterile operating room) and the auxiliary laboratory (the cytology laboratory, the biochemical analysis room, the photography room and the dark room). Conventional graphic design partitions are mainly divided into six areas according to the needs of the work content: aseptic operation, incubation, preparation, cleaning, disinfection and sterilization, and storage. Depending on the type of function, different devices need to be configured. The object of tissue cell culture technology research is a biological unit with complete vitality, so there are strict requirements for aseptic operation to avoid the influence of microorganisms and other harmful factors. The common instruments and equipment used in the preparation of cell laboratories mainly include: microscopes, incubators, drying boxes, water purification devices, refrigerators, cell freezers, centrifuges, balances, sterilizers, and filters.

According to user needs, CEIDI has compiled a list of commonly used instruments and equipment in cell laboratories and related operating instructions. The contents are as follows:

Common equipment 1: Microscope

The most common equipment in the cell laboratory is an inverted microscope, whose function is to grasp the growth of cells and observe whether they are contaminated. If the budget is sufficient, it can also be equipped with phase contrast microscopes, dissecting microscopes, fluorescence microscopes, and video recording systems to better observe, record, and photograph the growth of cells.

Common equipment 2: incubator

If it is necessary to culture the experimental cells in the cell laboratory in vitro, the relevant cells must be put into the incubator. Because in most cases, the most suitable temperature for cell survival is 37°C (temperature difference ±0.5°C). If the temperature is increased by 2°C, the cells can only survive for a few hours, and if it reaches 40°C, the cells will die quickly. At present, the most common incubator in cell laboratories is a CO2 incubator, which has the advantage of being able to provide quantitative CO2 (concentration of 5%), keeping the pH of the culture fluid stable, and suitable for open or semi-open culture. Place a petri dish, culture plate or culture bottle for cell culture in the incubator. If a culture bottle is to be used, it is recommended to loosen the cap slightly to ensure aeration.

Common equipment 3: Drying box

The relevant instruments and utensils of the incubator need to be disinfected and dried before they can be used. Therefore, the cell laboratory must be equipped with a drying box. The common drying oven is a blast-type electric heating drying oven, which has the advantages of uniform temperature and better drying effect. As for the use of the drying box, blasting and heating must be carried out at the same time. Stop blasting at 100°C to prevent the paper or cotton wrapping the utensils from being burnt and causing pollution. Do not open the door of the drying oven immediately after disinfection, but wait a few minutes to avoid damage to the utensils.

Common equipment 4: water purification device

Cell culture has high water quality requirements, so related water must be treated by a water purification device. Taking the preparation of various culture media and reagents for cell culture as an example, it is necessary to use distilled water three times, even if it is to rinse the utensils, use distilled water twice.

Common equipment 5: refrigerator

Refrigerators in the cell laboratory are divided into two types: ordinary refrigerators or refrigerators, low temperature refrigerators and ultra-low temperature refrigerators. Among them, the former is used to store culture fluid, physiological saline and related culture items and short-term preservation of tissue specimens. The latter is used to store preparations that require cryopreservation of biological activity and long-term storage, such as enzymes, serum, etc.

Common equipment 6: cell cryo storage

The cell freezing storage device mentioned here is a liquid nitrogen container, and its function is to freeze and store the cells required for the experimental project. When choosing a liquid nitrogen container, you need to consider three aspects, namely: volume, easy access and use, and liquid nitrogen volatilization. The size of the liquid nitrogen container is about 25L-500L, and it can store about 250-15000 ampoules of 1ml. Since the temperature of liquid nitrogen is -196°C, be careful when using it to avoid frostbite. In addition, due to the continuous volatilization of liquid nitrogen, attention should be paid to the situation of liquid nitrogen retention. If the amount of liquid nitrogen is insufficient, it should be supplemented in time to avoid excessive volatilization and cell damage.

Common equipment 7: centrifuge

Cell culture is usually divided into three tasks, namely: preparing cell suspension, adjusting cell density, washing and collecting cells. These three tasks are inseparable from the centrifuge, so it is also an indispensable instrument in the cell laboratory. If the cell laboratory has no special requirements, a 4000rpm benchtop centrifuge can be configured. If there is a need for cell sedimentation, a centrifuge of 80-00g can be configured. If there is a need for gradient centrifugation, a centrifuge with this function can be configured. In addition, a centrifuge with large capacity or adjustable temperature can also be configured as required.

Common equipment 8: balance

The balance in the cell laboratory includes a torsion balance and a precision balance. As for how to choose, it depends on the amount of weighing and weighing accuracy. If the sampling volume is more than 100 mg, use a balance with a sensitivity of 0.1 mg. If the sampling volume is 10mg-100mg, use a 0.01mg balance. If the sampling volume is less than 10 mg, use a 0.001 mg balance.

Common equipment 9: Sterilizer

As long as the items that come into contact with cells, whether in direct or indirect contact, they must be sterilized by a sterilizer. It is recommended that the cell laboratory be equipped with an autoclave sterilizer—pulsation vacuum sterilizer, which is characterized by the ability to monitor the pressure and temperature in the sterilization container while sterilizing and sterilizing, and to change various parameters through the memory support system To ensure the quality and safety of sterilization.

Common equipment 10: filter

The culture solution used for cell culture needs to be sterilized through a filter. There are 3 kinds of filters that are commonly used at present, namely: Zeiss filter, glass filter, and microporous filter.

In fact, in addition to the corresponding instruments and equipment, different laboratories also need to follow the relevant standards and combine the experimental functions and the operating habits of the users to design and build. The equipment of different configurations of laboratory functions will be different. The above instruments and equipment are conventional and basic configurations. CEIDI reminds companies that have laboratory construction needs to find experienced companies in the laboratory construction stage. In addition to meeting the needs, companies can issue suitable design plans and complete construction projects that meet standards. In addition, companies with a complete supply chain can also provide related laboratory furniture, equipment and other services.