NGS laboratory is mainly used for genetic testing, to be precise, high-throughput sequencing testing, and NGS is the abbreviation of "Next Generation Sequencing". This article will explain the basic requirements of NGS laboratory construction in detail.

Generally speaking, the basic requirements of NGS laboratory construction are mainly embodied in 7 aspects, namely: plane layout, main structure, division of various areas and air pressure adjustment, ground, disinfection, transmission window, and lighting.

1. Layout: The layout of the NGS laboratory should be considered from the three aspects of testing items, testing technology platforms, and specific workloads, and must comply with the principles of "orderly work, non-interference, prevention of pollution, and timely reporting".

2. Main structure: The main materials used in the construction of the NGS laboratory are color steel plates and aluminum alloy materials. The internal and external corners of the room are made of aluminum alloy with 50 internal rounded aluminum, which can not only ensure a strong indoor structure and good sealing. , And it is not easy to be polluted and dusty.

3. Division of each area and air pressure adjustment: Let's look at the division of each area first. The NGS laboratory is roughly divided into four areas: reagent preparation, sample preparation, PCR amplification, and high-throughput sequencing. Let's look at the air pressure adjustment again. It is necessary to install return air vents with adjustable air volume on the outer walls and doors of the experimental area, and the air is ventilated into the room through the return air vents.

4. Floor: The floor of the NGS laboratory must be corrosion-resistant, easy to clean, and have good integrity. Therefore, PVC coiled floor or artesian floor is a good choice. If the budget is limited, it is recommended to choose terrazzo floors or large tiles.

5. Disinfection: UV lamps for disinfection should be provided in the experimental area, the top of the buffer zone, and inside the transmission window of the NGS laboratory. If local disinfection of the laboratory table is to be carried out, a mobile ultraviolet lamp is also required.

6. Transfer window: There are two types of transfer windows: mechanical interlocking type and electronic interlocking type. NGS laboratory recommends a mechanical interlocking type transfer window to ensure that samples and reagents are not contaminated during the transfer process.

7. Lighting: Due to the cleanliness requirements in the NGS laboratory, relevant lighting equipment must be dust-free and easy to clean. It is recommended to equip with purification lamps.