What are the main points of cell laboratory construction? What are the main points of cell laboratory management? If you still don’t know much about it, you might as well take a look at the following detailed explanation of the key points of cell laboratory construction and management, I believe it will be helpful to you.

I. Key points of cell laboratory construction

The main points of the cell laboratory construction are reflected in two aspects, namely: the area and room arrangement of the culture room, and the environment and facilities of the culture room.

①The area of the cultivation room and the arrangement of the room: the area of the cultivation room is related to the number of staff, the number of equipment and instruments, and the size of the laboratory and clean benches. In terms of room arrangement, a buffer room larger than 6 square meters should be set up. In addition, at least two exits must be established.

②Cultivation room environment and facilities: The room should be airtight, and it is not suitable to open external windows; doors, windows, and wall surfaces should use easy-to-clean and corrosion-resistant paint; equipped with air cleaning devices and fluorescent lamps; equipped with special power transformers for power supply , Improve the quality and reliability of power supply.

II. Key points of cell laboratory management

The main points of cell laboratory management are embodied in 4 aspects, namely: disinfection and cleaning management, incubator and clean bench management, liquid nitrogen bottle and low-temperature refrigerator management, and laboratory data management.

① Disinfection and cleaning management: The staff must first enter the buffer room, and then change into clean work clothes before entering the main laboratory; the first person entering and the last person leaving the main laboratory must turn on and off the air cleaning device; Perform ultraviolet disinfection for 1 hour; once a week for sanitation and disinfection, and once a month for comprehensive cleaning.

②Incubator and clean bench management: In the incubator, according to the types of cultured cells, the incubator is divided into corresponding areas; the carbon dioxide pressure gauge is observed every day to replace the carbon dioxide cylinder in time. In terms of clean workbench, the workbench should be divided into material area, operation area, and dirt area; UV disinfection is carried out for 1 hour before use, and after use, clean work must be done.

③Management of liquid nitrogen bottles and low-temperature refrigerators: Regarding liquid nitrogen bottles, the capacity of liquid nitrogen is regularly checked, and relevant records are made; it is stored in different boxes according to the type of cell line, and the name and time are marked and recorded. For low-temperature refrigerators, different areas should be divided according to their types, marked and recorded.

④Laboratory data management: do a good job of collecting various experimental data, experimental routes, operating procedures, and operating records; the data after the data is archived shall be managed by dedicated personnel; intellectual property issues such as patents and inventions should be kept confidential.