For the microbiology laboratory CEIDI Xidi has done a lot of science popularization, this article emphasizes the three aspects of the microbiology laboratory's composition, layout and common parameters. Microbiology laboratory generally consists of five parts: preparation room, ordinary laboratory, sterilization room, constant temperature culture room (such as bacteria culture room, mold culture room, liquid culture room, solid culture room, etc.) and sterile room. According to the field of work (food, medical, pharmaceutical) and nature (teaching, research, production, monitoring, etc.), the decoration design composition and scale of the microbiology laboratory are also different, and they cannot be the same. In this article, CEIDI will combine the past experience in microbiological design and decoration to explain in detail the construction specifications of the general functions of the microbiology laboratory. In addition, it is important to note that the sterile room and ultra-clean workbench are the core parts of the laboratory, which mainly provide protection for samples and ensure the accuracy of experimental results.

1. The composition of the microbiology laboratory

Microbial experiment is mainly aimed at the separation, cultivation, breeding, identification, morphology, variation, utilization, and harm of microorganisms. The decoration design of the microbiology laboratory generally includes: preparation room, microbial culture room, equipment disinfection and cleaning room, pure water room, testing room, bacteria room, storage room, etc.

2. The layout of the microbiology laboratory

The microbiology laboratory should be set up as an independent area, separated from other laboratories, the door should be equipped with access control, non-related personnel should not enter, and each room should be reasonably arranged according to the work content, which is convenient for work and does not affect each other.

1. Preparation room

The preparation room is used to prepare culture media and sample processing. Mainly equipped with laboratory benches, counters, refrigerators, electric stoves and power supplies, water pipes, heaters, steamers, washing utensils, washing agents, etc.

2. General laboratory

Ordinary laboratories are used for the observation, counting and physiological and biochemical determination of microorganisms. The layout of an ordinary laboratory is determined by the actual work requirements of the construction party, and is generally equipped with microscopes, laboratory benches, reagent cabinets, etc.

3. Sterilization room

The sterilization chamber is used to sterilize the culture medium and various utensils. Mainly equipped with high pressure steam sterilizer, oven, etc.

4. Constant temperature culture room

① Cultivation room design: The culture room should have an inner and an outer room. The inner room is the culture room and the outer room is the buffer room. The center of the inner and outer rooms should be equipped with ultraviolet lamps for sterilization; a constant temperature and humidity machine should be installed to maintain Indoor temperature and humidity requirements; vents with air filtering devices should be installed on the partition walls of the inner and outer rooms.

② Cultivation room equipment: the inner room is equipped with a culture rack and a shaker (shaker); the outer room is equipped with a full set of special work clothes, a hand-held sprayer, 5% carbolic acid solution, 70% alcohol cotton balls, etc.

5. Clean room

The inoculation, transplantation and purification of strains is an important operation in microbiological experiments. The sterile room is set up to ensure pure strains and prevent contamination of bacteria.

① The sterile room should have an inner and an outer room, an inner sterile room and an outer buffer room. The internal and external volume should be small rather than large to facilitate air sterilization.

② Ventilation windows should be installed above the entrance of the inner compartment, and constant temperature and humidity machines can also be installed if conditions permit. The ventilation window is preferably a double-layer structure, the outer layer is a shutter, and the inner layer can be a sash window.

③ Passing windows can be installed on the partition wall between the inside and outside to reduce personnel entry and exit and reduce pollution.

3. Parameter settings among some important functions of the microbiology laboratory

1. Identify the environmental temperature and humidity requirements of various tasks. The main considerations are: instruments, reagents, experimental procedures, laboratory staff (the human body generally feels comfortable at a temperature of 18-25 ℃ and a relative humidity of 35-80%, and from a medical point of view, the environment is dry and the throat inflammation is certain Causality).

2. Maintain and monitor. Various measures are taken to ensure that the temperature and humidity of the environment are within the control range, and the environmental temperature and humidity are monitored and recorded, and measures are taken in time if the allowed range is exceeded, the air conditioner is turned on to adjust the temperature, and the dehumidifier is opened to control the humidity.

3. Select and develop an effective environmental temperature and humidity control range. Extract the narrowest range from the list of requirements for the above elements as the allowable range of environmental control for the laboratory, formulate management procedures for environmental condition control, and formulate reasonable and effective SOPs based on the actual situation of the department.

4. Common parameter settings:

① Infrared room temperature 10-30℃, humidity 35-60%

② Water room temperature 10-30℃, humidity 35-65%

③ Sample room temperature 10-25℃, humidity 35-70%

④ Reagent room temperature 10-30℃, humidity 35-80%

⑤ Balance room temperature 10-30℃, humidity 35-80%

⑥ Central laboratory temperature 10-30℃, humidity 35-80%

⑦ Sample storage room temperature 10-30℃, humidity 35-80%

Four, Xidi Microbiology Laboratory Decoration Design Case

CEIDI, as an outstanding representative of Shanghai laboratory and clean room engineering EPC, has been deeply involved in the construction of laboratories, clean workshops and other clean engineering fields, creating a high-quality, safe and sustainable experimental environment for the majority of laboratory employees. In July 2019, the "Baiqu Medical In Vitro Diagnostic Kit Pilot Base Renovation Project" contracted by CEIDI Xidi started construction in Jiading, Shanghai. This pilot-scale test base for medical testing products of more than 1,000 square meters is responsible for important clinical scientific research before the "research grade" medical testing products are put into production in batches.